|
Subunit: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain) Miscellaneous: Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form Sequence caution: Sequence=AAK28401.1; Type=Frameshift; Positions=108; Sequence=BAA24843.2; Type=Erroneous initiation; Selected PDB 3D structures from [IMAGE] and Proteopedia [IMAGE] for APAF1 (see all 8): 1C15 (3D) [IMAGE] 1CWW (3D) [IMAGE] 1CY5 (3D) [IMAGE] 1Z6T (3D) [IMAGE] 2P1H (3D) [IMAGE] 2YGS (3D) [IMAGE]
Tissue specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver [IMAGE] Pathway & Disease-focused RT2 Profiler PCR Arrays including APAF1 (see
Function: UniProtKB/Swiss-Prot Summary: APAF_HUMAN, O14727 Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis
Similarity: Contains 1 CARD domain Contains 1 NB-ARC domain Contains 15 WD repeats [IMAGE]
|
