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Subunit: Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA
Tissue specificity: Expressed in all adult tissues examined except for lung and kidney [IMAGE] Pathway & Disease-focused RT2 Profiler PCR Array including LATS1: Hedgehog Signaling Pathway in human mouse rat Primer Products: [IMAGE] OriGene qSTAR qPCR primer pairs in human, m
Function: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function
Catalytic activity: ATP + a protein = ADP + a phosphoprotein Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112 Gene Ontology (GO): Selected molecular function terms (see all 8): About this table GO ID Qualified GO term Evidence PubMed IDs GO:0000287 magnesium ion binding IDA 10518011 GO:0004672 protein kinase activity -- -- GO:0004674 protein serine/threonine kinase activity TAS -- GO:0004713 protein tyrosine kinase activity -- -- GO:0005515 protein binding IPI 10831611 [IMAGE] Find genes that share ontologies with LATS1 About GenesLikeMe Phenotypes: 3 GenomeRNAi human phenotypes for LATS1: Decreased focal adhesion (FA) Decreased sensitivity to pacli Decreased substrate adherent c 11 MGI mutant phenotypes (inferred from 3 alleles[IMAGE]) (MGI details for Lats1): cardiovascular system cellular endocrine/exocrine gland growth/size/body homeostasis/metabolism integument mortality/aging nervous system no phenotypic analysis reproductive system tumorigenesis [IMAGE] Find genes that share phenotypes with LATS1 About GenesLikeMe Animal Models: MGI mouse knock-outs for LATS1: Lats1tm1Shku Lats1tm1Noj Lats1tm1Tx [IMAGE] inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for LATS1 [IMAGE] inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for LATS1 [IMAGE] genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for LATS1 [IMAGE] genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for LATS1 miRNA Products: miRTarBase miRNAs that target LATS1: hsa-mir-103a-3p (MIRT027020), hsa-mir-335-5p (MIRT016931), hsa-mir-21-5p (MIRT030710), hsa-mir-16-5p (MIRT031514) [IMAGE] Block miRNA regulation of human, mouse, rat LATS1 using miScript Target Protectors [IMAGE] Selected qRT-PCR Assays for microRNAs that regulate LATS1 (see all 22): hsa-miR-3607-3p hsa-miR-624* hsa-miR-944 hsa-miR-548a-3p hsa-miR-511 hsa-miR-548g hsa-miR-204 hsa-miR-3143 [IMAGE] SwitchGear 3'UTR luciferase reporter plasmid: LATS1 3' UTR sequence Inhib. RNA Products: [IMAGE] OriGene RNAi products in human, mouse, rat for LATS1 [IMAGE] Predesigned siRNA for gene silencing in human, mouse, rat LATS1 Gene Editing Products: [IMAGE] DNA2.0 Custom Protein Engineering Service for LATS1 Clone Products: [IMAGE] OriGene clones in human, mouse for LATS1 (see all 7) OriGene ORF clones in mouse, rat for LATS1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling [IMAGE] GenScript: all cDNA clones in your preferred vector: LATS1 (NM_004690) [IMAGE] Sino Biological Human cDNA Clone for LATS1 [IMAGE] DNA2.0 Custom Codon Optimized Gene Synthesis Service for LATS1 [IMAGE] Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat LATS1 [IMAGE] Addgene plasmids for LATS1 [IMAGE] Cell Line Products: [IMAGE] GenScript Custom overexpressing Cell Line Services for LATS1 [IMAGE] Browse ESI BIO Cell Lines and PureStem Progenitors for LATS1 [IMAGE] In Situ Assay Products: [IMAGE] Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for LATS1
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family Contains 1 AGC-kinase C-terminal domain Contains 1 protein kinase domain Contains 1 UBA domain [IMAGE]
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